Research Paper Volume 11, Issue 21 pp 9672—9688
Glycogen synthase kinase-3β suppresses the expression of protein phosphatase methylesterase-1 through β-catenin
Figure 1. GSK-3β suppresses the expression of PME-1. (A) HEK-293T cells were transfected with pCI/HA-GSK-3β and the levels of GSK-3β, demethylated PP2Ac and PME-1 were analyzed by Western blots. (B–D) SH-SY5Y cells were transfected with pCI/HA-GSK-3β. The mRNA level of PME-1 was analyzed by qPCR (C). The protein levels of PME-1 and GSK-3β were analyzed by Western blots (B) and normalized with GAPDH (D). (E–G) SH-SY5Y cells were transfected with siGSK-3β to knockdown the expression of GSK-3β. The protein levels of PME-1 and GSK-3β were analyzed by Western blots (E) and normalized with GAPDH (G). The mRNA level of PME-1 was measured by qPCR (F). (H) SH-SY5Y cells were transfected with siGSK-3α to knockdown the expression of GSK-3α. The protein levels of PME-1 and GSK-3α/β were analyzed by Western blots. Data are presented as mean ± SD (n=3), *P < 0.05, ***P < 0.001.