Research Paper Volume 11, Issue 21 pp 9369—9387

Figure 5. Lifespan extension by KRG is mediated through the IIS pathway. (A) mRNA levels of dilps and target genes of dFOXO were analyzed in fruit flies fed a KRG-containing diet or a control diet for 7 or 40 days. *p < 0.01. (B) Translocalization of dFOXO to the nucleus following supplementation of diet with KRG. The abdominal fat body was stained with anti-dFOXO (red) and DAPI (blue). Original magnification is 200×. (C) The level of phosphorylated Akt (pAkt)/Akt of flies fed a KRG-containing diet (lane 4, 5, 6) or a control diet (lane 1, 2, 3). (D) Survival of heterozygous mutant chico flies (chico^1/+, dashed lines) and control flies (w¹¹¹⁸, solid lines) fed a KRG-containing diet (lines with open dots) or a control diet (lines with closed dots). (E) Survival of transheterozygous mutant dFOXO flies (foxo^21/25, dashed lines) and control flies (yw, solid lines) fed a KRG-containing diet (lines with open dots) or a control diet (lines with closed dots).