Figure 7.Overexpression of TUG1 promotes sensitivity of NSCLC cells by positively regulating PTEN. (A) colony formation assay to detect the effect of TUG1 and PTEN on cell colony formation ability; (B) scratch test to detect the effect of TUG1 and PTEN on cell migration ability; (C) Transwell invasion assay to detect the effect of TUG1 and PTEN on cell invasion ability; (D) flow cytometry to detect the effect of TUG1 and PTEN on cell cycle; (E) flow cytometry to detect the effect of TUG1 and PTEN on cell apoptosis; (F) expression of autophagy-related proteins and cell senescence-related protein hTERT determined by Western blot analysis, normalized to β-actin. * p < 0.05 versus the NC group; ** p < 0.01.
