Research Paper Volume 11, Issue 18 pp 7402—7415

Figure 2. IL-1β and TNF-α upregulate CCL28 through activating NF-κB after SCI. (A, B) Mice were pre-injected with control antibody (Ctrl Ab) or neutralizing antibodies against IL-1β (anti-IL-1β) and/or TNF-α (anti-TNF-α) into the intraspinal cord for 12 hrs, and then subjected to sham or SCI surgery. After another 12 hrs, the spinal cord samples were analyzed by qRT-PCR and Western blotting for detecting CCL28 mRNA level (A) and protein level (B) (n=5). (C, D) Mice were pre-injected with equal volume of vehicle or 60 mg/kg ML120B into the intraspinal cord for 12 hrs, and then subjected to sham or SCI surgery. After another 12 hrs, the CCL28 mRNA level (C) and protein level (D) in the spinal cord was analyzed as in (A, B) (n=5). GAPDH was used as a reference or loading control. Data are mean ± SD. The statistical analysis was performed using Student’s t-test. **, P<0.01; NS, not significant.