Research Paper Volume 11, Issue 15 pp 5483—5497

Figure 4. Western blotting (WB), quantitative real-time PCR (qPCR), and immunohistochemistry (IHC) measurements of CBX3/HP1γ in hepatocellular carcinoma (HCC) tissues and HCC tissue microarrays. (A and B) WB results show that CBX3/HP1γ expression was higher in tumor tissues than in adjacent normal tissue. (C) qPCR confirmed that CBX3/HP1γ expression was higher in tumor tissues. (D and E) IHC for CBX3/HP1γ protein in HCC tissue microarrays. (D) Representative image of strong CBX3/HP1γ staining (high expression) in tumor cell nuclei (left ×40, right ×400). (E) Representative image of weak CBX3/HP1γ staining (low expression) in tumor cell nuclei (left ×40, right ×400). (F) CBX3 expression was negatively correlated with overall survival (r = -0.139, P = 0.0088). (G) High CBX3/HP1γ expression was associated with worse overall survival (OS) in HCC patients (Kaplan- Meier analysis, log-rank test, P value = 0.008). (H) High CBX3/HP1γ expression was associated with worse recurrence free survival (RFS) in HCC patients (Kaplan- Meier analysis, log-rank test, P value = 0.041).