Research Paper Volume 11, Issue 11 pp 3768—3784

Figure 1. The effect of changes in the expression of MEF2A on the HUVEC phenotype. (A) The images show the SA-β-galactosidase staining positive cells (blue) in NC and MEF2A-specific siRNA (si-MEF2A) group, and the histogram shows the fold changes of the senescence rate relative to NC group. (B) The images show the SA-β-galactosidase staining positive cells in control plasmid (pc3-gab), wild type MEF2A overexpression plasmid (pc3-gab-MEF2A-2) and frameshift mutant MEF2A plasmid (pc3-gab-MEF2A-MV30) group, and the histogram shows the fold changes of the senescence rate relative to pc3-gab group. (C) Fold changes of cell viability relative to NC group. (D) Fold changes of cell viability relative to pc3-gab group. The number of total cells and SA-β-gal staining positive cells were counted in 20-30 images photographed from different fields of view in the duplicate wells in each experiment. The senescence rate was calculated by dividing the number of SA-β-gal staining positive cells by the number of the total cells. The histograms show the mean ± SEM of more than three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.