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Research Paper Volume 11, Issue 11 pp 3668—3678
Apigenin inhibits growth and migration of fibroblasts by suppressing FAK signaling
Figure 1. Apigenin inhibits the cell survival and migration of HSFs. (A) Cell clone formation assays. The results shown depict the mean±SD of three experiments done in triplicate. **P < 0.05 vs. NaCl. (B) MTT assays of HSFs and HKFs cultured with indicated concentrations of apigenin. Results depict the mean±SD of three experiments done in triplicate. **P < 0.05 vs. NaCl. (C) IC50 values for the inhibitory effects of apigenin on HSFs and HKFs were calculated from the linear curve fitted to the MTT data. (D, E) FACS analysis of HSFs and HKFs pre-treated with the indicated concentrations of apigenin. The experiment was repeated three times. **P < 0.05 vs. NaCl. (F–I) Transwell assays showing the effects of pretreatment with apigenin for 24 h on HSF and HKF migration, with and without Matrigel. Migrated cells were counted, and the results depict the mean±SD of three experiments. **P < 0.05 vs. NaCl.