Research Paper Volume 8, Issue 10 pp 2568—2589

Figure 3. The long-lived mutant strains 3, 5 and 12 do not differ from the parental WT strain in efficacy of their sexual reproduction by sporulation, a measure of fecundity and a key trait of early-life fitness. Each of the four diploid strains formed between cells of the haploid WT strain BY4741 (MATa) and cells of the haploid WT strain BY4742 (MATα) or cells of each of the selected long-lived haploid mutant strains 3, 5 or 12 (each MATα in the BY4742 genetic background) were pre-grown to mid-logarithmic phase in YP medium initially containing 0.2% glucose (a fermentable carbon source; CR conditions) (A) or 1% ethanol (a non-fermentable carbon) (B). The efficiency of sporulation of each of the four diploid strains was measured at various time points since the beginning of a sporulation assay as described in the ″Materials and methods″ section; it was calculated as the percentage of tetrads and dyads produced by a diploid strain, relative to the total number of cells. Data are presented as means ± SEM (n = 3; ns, not significant difference). At each time point, sporulation efficiencies of the WT × 3, WT × 5 and WT × 12 diploid strains were statistically insignificant in comparison with sporulation efficiency of the WT × WT diploid strain.