Research Paper Volume 8, Issue 10 pp 2551—2567
Decreased mTOR signalling reduces mitochondrial ROS in brain via accumulation of the telomerase protein TERT within mitochondria
Figure 4. TERT protein is required for the reduction of H2O2 release from mouse brain mitochondria by rapamycin. (A) Mouse TERT protein abundance in brain homogenates and isolated mitochondria after 4 months rapamycin treatment. (n=5 per group, t-test, ***p<0.001). (B) H2O2 release (as percentage of controls) from brain mitochondrial complex I (maximum capacity, with complex I-linked substrate, pyruvate plus malate in the presence of rotenone) from wild-type (WT) or first generation TERT-/- mice fed either control or rapamycin-containing diet for 4 months. (n=4-5 per group, t-test: *P<0.05) C: H2O2 release from brain mitochondria as measured in (B), but with the complex II-linked substrate, succinate (4mM). n=4-5 mice per group, t-test *P<0.05.