Research Paper Volume 8, Issue 10 pp 2463—2487

Figure 6. PRG3 impedes lysophosphatidic acid (LPA) induced axon collapse. (A) Live time collapse assay with axon collapse inducing LPA. Representative time laps microscopy image series of GFP and PRG3 expressing neurons treated with 0.5 µM LPA. Transfection efficacy is shown in first image bottom right corner. Scale bar represents 50 µm. (B) Quantification of neurite length of neurons expressing GFP (control) or PRG3. Note, that GFP expressing neurons show significant higher number of retracted neurites and round cell bodies during the time lapse assay. Three independent experiments were carried out and differences were considered statistically significant with * p<0.05, ** p<0.01, *** p<0.001 (two-tailed t-test). Values are given as mean ± SEM.