Research Paper Volume 8, Issue 10 pp 2337—2354
TGF-β and NF-κB signaling pathway crosstalk potentiates corneal epithelial senescence through an RNA stress response
Figure 4. Effects of TGF-β1 on SASP production. (A) mRNA levels of the indicated genes in HCECs treated with TGF-β1 (10 ng/ml) for 3days. (B) The IL-6 and IL-8 in cultured HCECs supernatants were detected by ELISA. (C) HCECs were stimulated with TGF-β1 (10 ng/ml) for 24 h. NF-kB p65 translocation was detected by fluorescent microscope. (D) HCECs were treated with TGF-β1 (10 ng/ml) for 24 h. Cytoplasmic and nuclear levels of NF-kB p65 were analyzed by Western blot. (E) Western blots of whole cell extraction from HCECs treated with TGF-β1 (10 ng/ml) for 24h. (F) Quantification of western blots processed with Image J. **P≤0.01. Data are representative of three independent experiments.