Research Paper Volume 8, Issue 10 pp 2308—2323

Figure 9. SIRT6 represses cellular senescence induced by p27. (A) 2BS cells were stably co-transfected with LPC vector and pBabe vector, LPC vector and pBabe-p27 or LPC-SIRT6 and pBabe-p27 plasmids. The expression of SIRT6 and p27 protein levels in the stable transformants were analyzed by western blot. (B) Cell cycle of cells from A was detected. Values represent the means ± S.E. of triplicate points from a representative experiments (n=3), which was repeated three times. (C) Cells from A were stained for SA-β-gal activity.