Research Paper Volume 8, Issue 9 pp 1876—1895

Figure 6. Lin-42 regulates lifespan and autophagy in C. elegans. Survival analyses of wild-type animals (WT) compared to lin-42(n1089) mutants (A) or to lin-42 (+) transgenic animals (B). Survival analyses of wild-type animals (WT) and lin-42 (+) transgenic animals fed bacteria transformed with empty-vector (con) or with vector expressing dsRNA against bec-1 (C) or atg-5 (D). (E) Table summarizing survival data analysis from (A-D). (F) Quantification of LGG-1/LC3::GFP positive foci in the DA2123 transgenic strain fed bacteria transformed with empty-vector (con) or with vector expressing dsRNA against lin-42 (lin-42 RNAi). Results are plotted as mean ± SEM of GFP foci (***) represent the P-value (<0.0001) calculated by performing the t-test between the 2 conditions. (G) P62/SQST-1::GFP translational reporter strain (HZ589) fed bacteria transformed with empty-vector (con) or with vector expressing dsRNA against lin-42 (lin-42 RNAi). Top panels: green fluorescence channel (GFP) images. Bottom panels: differential (Nomarski) interference contrast images (DIC). Red squares indicate selected areas used for fluorescence foci quantification. White bars = 20µm. (H) Quantification of GFP positive foci in the anterior pharyngeal bulb of HZ589 fed bacteria transformed with empty-vector (con) or with vector expressing dsRNA against lin-42 (lin-42 RNAi). Results are plotted as normalized mean ± SEM of GFP foci, relative to the control. (*) represent the P-value (<0.05) calculated by performing the t-test between the 2 conditions.