Research Paper Volume 8, Issue 8 pp 1759—1780
Scavengers of reactive γ-ketoaldehydes extend Caenorhabditis elegans lifespan and healthspan through protein-level interactions with SIR-2.1 and ETS-7
Figure 4. SA treatment dose-dependently decreases biomarkers of oxidant injury in a SIR-2.1-dependent manner. (A, B) Quantification of oxidant damage via F3-IsoP measurement. N2 WT and SIR-2.1 mutant animals were given SA from day 1 of adulthood until collection. Lysates were collected at day 15 of adulthood and F3-IsoPs were measured by GC/MS. Data are expressed as means ± SEM from four independent experiments. * P < 0.01 as compared with vehicle control, **P < 0.005 as compared to vehicle control. (C) Levels of acetyl-Lys 122 MnSOD was measured from N2 WT and SIR-2.1 mutant protein extracts and analyzed by Western blot. (D) Quantification of acetyl-Lys 122 MnSOD. Treatment with SA in WT N2 nematodes show a trend toward lower acetyl-Lys 122 MnSOD compared to SIR-2.1 mutant animals. Data are expressed as means ± SEM from four independent experiments. P > 0.05.