Research Paper Volume 8, Issue 8 pp 1759—1780
Scavengers of reactive γ-ketoaldehydes extend Caenorhabditis elegans lifespan and healthspan through protein-level interactions with SIR-2.1 and ETS-7
Figure 2. SA administration decreases formation of IsoK-lysyl-lactam protein adducts. (A) Schematic illustrating lipid peroxidation and formation of IsoKs. Isoks react with ε-amino in lysyl residues of proteins to form stable lactam adducts. Addition of the IsoK scavenger, SA, prevents IsoK adduction. (B) IsoK-lysyl-lactam adduct quantification by LC/MS/MS. IsoK-lysyl-lactam adducts were decreased with SA treatment. Data are expressed as means ± SEM from four independent experiments. *P < 0.01 as compared with vehicle control, **P < 0.005 as compared to vehicle control.