Research Paper Volume 8, Issue 8 pp 1718—1734
Biological and biophysics aspects of metformin-induced effects: cortex mitochondrial dysfunction and promotion of toxic amyloid pre-fibrillar aggregates
Figure 5. Metformin induces apoptosis mainly in neurons of cortex region. TUNEL assay was performed on the paraffin sections of cortex and hippocampus obtained from mice untreated or treated with metformin to detect apoptotic nuclei (brown). (a) Representative cortex and hippocampus brain sections stained with TUNEL in control and metformin treated mice. High magnification of the squared areas is shown. (b) The histogram indicates the percentage of positive cells both in cortex and hippocampus regions normalized to the control. 10X original magnification. Scale bars = 100 μm. n=5 per group