Research Paper Volume 8, Issue 7 pp 1442—1456
miR-638 suppresses DNA damage repair by targeting SMC1A expression in terminally differentiated cells
Figure 3. SMC1A and BRC1A expression levels are down-regulated in terminally differentiat-ed cells. (A) The mRNA and protein levels of SMC1A were measured by qRT-PCR and western blotting, respectively, in PMA-treated K562 cells, PMA-treated HL-60 cells, and DMSO-treated HL-60 cells. (B) The mRNA and protein levels of BRCA1 were measured by qRT-PCR and western blotting, respectively, in PMA-treated K562 cells, PMA-treated HL-60 cells, and DMSO-treated HL-60 cells. Each group of comparative qRT-PCR contained three duplicates, and the results were normalized to GAPDH mRNA. HSC70 served as the loading control. **: p<0.01, ***: p<0.001, #: p<0.0001, compared to control with the Student's t test. Error bars, S.D.