Research Paper Volume 8, Issue 7 pp 1442—1456

Figure 1. The expression of miR-638 is up-regulated in terminally differentiated cells. (A) K562 cells were treated with or without phorbol myristate acetate (PMA) for 96 h, after which they were stained with a fluorescence-conjugated antibody against CD41a. The expression of CD41a was detected by flow cytometry. Levels of miR-638 in treated and untreated cells were detected by qRT-PCR. (B) HL-60 cells were treated with or without PMA for 72 h. Cells were then stained with a fluorescence-conjugated antibody against CD14, and the expression of CD14 was detected by flow cytometry. Levels of miR-638 in treated and untreated cells were detected by qRT-PCR. (C) HL-60 cells were treated with or without dimethyl sulfoxide (DMSO) for 120 h. Cells were then stained with a fluorescence-conjugated antibody against CD11b, and the expression of CD11b was detected by flow cytometry. Levels of miR-638 in treated and untreated cells were detected by qRT-PCR. The numbers of positively stained cells after PMA or DMSO induction are shown in the graphs. Comparative qRT-PCR was run in three duplicates per group, and the results were normalized to Snord44 snRNA. #: p<0.0001, compared to control with the Student's t test. Error bars, S.D.