Research Paper Volume 8, Issue 7 pp 1384—1397

Figure 4. FOXM1 levels decrease during keratinocyte senescence. (A)NHEKs were maintained for 4 passages, and FOXM1, p63, Rb, and p16 levels were analyzed for each passage by western blot. (B)qPCR analysis of FOXM1 and p16 expression levels in proliferating (P1) and senescent (P4) keratinocytes. Values reported are the average ± SD of three independent experiments. **p-value <0.01 by Student's t-test (C)NHEKs were silenced for 96 h, after which SA-β-galactosidase assays were performed. Values reported are the average ± SD of two independent experiments. *p-value <0.005 by Student's t-test. (D) qRT-PCR analysis of FOXM1 and p63 expression levels in primary keratinocytes obtained from biopsies of young (<10 y.o.) and aged (>60 y.o.) donor skin. *p-value <0.005 by Student's t-test. (E)Western blot analysis of FOXM1 and p16 levels in keratinocytes grown as in (D).