Research Paper Volume 8, Issue 7 pp 1330—1349

Figure 7. Mitophagy deficiency preserves the pluripotency of iPSCs. Histological analyses of PINK1^+/+- and PINK1^−/−-iPSC-derived teratomas. Representative photographs of teratomas (circled with white dotted lines) are shown. PINK1^+/+-iPSC-derived teratomas: (a) Primitive neural tissue, (b) Primitive neural tissue and skeletal muscle tissue, (c) Squamous epithelium, immature neural and glial tissue, (d) Mature nervous tissue, squamous keratinized epithelium, skeletal muscle tissue, mucinous glands, (e) Squamous keratinized epithelium, skeletal muscle tissue, (f) Mature nervous tissue, (g) Squamous keratinized epithelium, skeletal muscle tissue, respiratory epithelium. PINK1^−/−-iPSC-derived teratomas; (a') Whole-tumor section with dark areas of primitive neuroepithelium mixed with skeletal muscle tissue and seromucinous glands, (b') Mature nervous tissue, (c') Skeletal muscle tissue, (d') Mature nervous tissue, squamous keratinized epithelium, skeletal muscle tissue, mucinous glands, (e') Mature nervous tissue, squamous keratinized epithelium, skeletal muscle tissue, (f') Mature nervous tissue, (g') Mature nervous tissue, mucinous glands, osteoid substance. Note that teratomas from PINK1^+/+-iPSCs and PINK1^−/−-iPSCs similarly show mixed tissues apparently derived from the three germ layers, i.e., ECT: Ectoderm, MS: Mesoderm, and END: Endoderm.