Figure 8.SIRT6 binds to and deacetylates H3K9 at TNFSF4 gene promoter(A) SIRT6 binds to TNFSF4 gene promoter in HUVECs. Sonicated chromatin from cultured HUVECs were used for ChIP assay, n=3. (B) SIRT6 deacetylates H3K9 at TNFSF4 gene promoter in HUVECs. HUVECs were infected with control adenovirus or SIRT6 adenovirus (M.O.I. 10) for 24 h. ChIP-qPCR was performed to determine SIRT6 and H3K9Ac occupancy at TNFSF4 gene proximal promoter. n=3, **P<0.01 versus Ad-ctrl. (C) SIRT6 inhibits basal and TNF-α induced TNFSF4 mRNA expression in HUVECs. HUVECs were infected with SIRT6 adenovirus (M.O.I 10) for 24 h before stimulation with TNF-α for 6 h, TNFSF4 mRNA was evaluated by q-RT-PCR after normalization with internal control, n=3, *P<0.05, ***P<0.001, compared to Ad-ctrl, ###P<0.001 compared to Ad-ctrl+TNF-α.
