Research Paper Volume 8, Issue 5 pp 1064—1078

Figure 8. SIRT6 binds to and deacetylates H3K9 at TNFSF4 gene promoter. (A) SIRT6 binds to TNFSF4 gene promoter in HUVECs. Sonicated chromatin from cultured HUVECs were used for ChIP assay, n=3. (B) SIRT6 deacetylates H3K9 at TNFSF4 gene promoter in HUVECs. HUVECs were infected with control adenovirus or SIRT6 adenovirus (M.O.I. 10) for 24 h. ChIP-qPCR was performed to determine SIRT6 and H3K9Ac occupancy at TNFSF4 gene proximal promoter. n=3, **P<0.01 versus Ad-ctrl. (C) SIRT6 inhibits basal and TNF-α induced TNFSF4 mRNA expression in HUVECs. HUVECs were infected with SIRT6 adenovirus (M.O.I 10) for 24 h before stimulation with TNF-α for 6 h, TNFSF4 mRNA was evaluated by q-RT-PCR after normalization with internal control, n=3, *P<0.05, ***P<0.001, compared to Ad-ctrl, ###P<0.001 compared to Ad-ctrl+TNF-α.