Research Paper Volume 8, Issue 5 pp 1064—1078

Figure 6. SIRT6 inhibits monocyte adhesion to endothelial cells by attenuating VCAM-1. (A-B) HUVECs transfected with SIRT6 siRNA (48 h, A) or infected with SIRT6 adenovirus (10 M.O.I., 24 h, B), and then monocyte adhesion to endothelial cells was determined. The representative images were shown after Image J adjustment of the threshold to label adhered monocytes with red color, original magnification, ×10. (C-D) Quantification of panel A and B, n=3-4. (E-F) HUVECs transfected with SIRT6 siRNA (48 h, E) or infected with SIRT6 adenovirus (10 M.O.I., 24 h, F) as indicated, then stimulated with TNF-α for 6 h, whole cell lysates were collected for Western blot analysis to detect VCAM-1 expression. GAPDH was used as the loading control, n=3.