Research Paper Volume 8, Issue 5 pp 1064—1078

Figure 4. SIRT6 haploinsufficiency exacerbates atherogenesis in ApoE^−/− mice. (A) Genotyping of SIRT6 and ApoE wild-type and mutant mice. SIRT6^+/−/ApoE^−/− mice were generated by cross-breeding male SIRT6^+/−/ApoE^+/− mice with ApoE^−/− females. Representative images show the results of genotypes by tail genomic DNA PCR method. SIRT6^−(Δ), and SIRT6^+(WT) bands appear at 524 bp and 390 bp, respectively. ApoE^−(Δ), ApoE^+(WT) bands appear at 245 bp and 155 bp, respectively. (B) SIRT6^+/−/ApoE^−/− developed more atherosclerotic plaques in en face aorta. SIRT6^+/−/ApoE^−/− and SIRT6^+/+/ApoE^−/− mice were fed HFD for 8 weeks. The mice were then sacrificed and aortas from the aortic arch to the iliac arteries were examined for atherosclerotic lesion formation using the en face Oil-Red O staining. The images presented were a composite of 4-6 images captured at different regions of the same aorta, scale bar=4 mm. (C) Percentage of atherosclerotic lesions in the whole aorta was quantified by Image-Pro Plus software, n=7. (D-E) SIRT6^+/−/ApoE^−/− developed more atherosclerotic lesions in the aortic sinus, n=9-10, scale bar=0.4 mm.