Research Paper Volume 8, Issue 6 pp 1223—1235

Figure 1. DCs from elderly are compromised in their ability to induce T regulatory cells after RA treatment. DCs from aged and young subjects were stimulated with RA and subsequently cultured with CD4 T cells. T cell priming was assayed. (A) Flow chart for experimental set up. (B) Dot plot depicts the percentage of CD4⁺CD25⁺CD127^lo FoxP3⁺ T reg cells after 5 days co-culture of DCs with CD4 T cells. Figures are representative of 10 such experiments with different aged and young subjects. (C) Bar graphs Mean +/− S.E. of the percentage of CD4⁺CD25⁺CD127^lo FoxP3⁺ T reg cells. Bar graphs depict the levels of cytokines secreted by T cells in the DC-T cell co-culture. (D) IL-10; E. IFN-γ. Figures are mean ⁺/− S.E. of 10 experiments with different aged and young subjects.