Figure 1.Experimental design of comprehensive regional and temporal profiling of murine brain proteome and metabolomePrior to brain sample collection the Focused Beam Microwave Irradiation (FBMI) was applied as a metabolism quenching technique to inactivate the enzymes and prevent the metabolite degradation during the post-mortem delay (See also Figure S1). Initially the hippocampus was dissected and extracted for proteome profiling using nano SWATH-MS – nano flow data independent tandem mass spectrometry acquisition mode with variable windows to facilitate protein quantification. As the altered hippocampal proteome implied the changes in metabolic processes, the metabolome profiling was performed on three different brain regions using micro HILIC-DDA-MS – capillary flow hydrophilic interaction chromatography coupled with data dependent tandem mass spectrometry acquisition mode to facilitate metabolite identification.
