Research Paper Volume 8, Issue 5 pp 1049—1060

Figure 5. The effects of age and stroke on post-stroke lymphocyte infiltration into the brain. (A) A significantly larger proportion of T cells infiltrating into the ischemic hemisphere 72 hours after 90 minute MCAO were BrdU-positive in aged mice compared to young (p<0.05)(n=4-6/group). (B) There was a significant effect of age (F(1,16)=p<0.001) on the number of T cells infiltrating into the brain 72 hours after 90 minute MCAO as measured by 2-way ANOVA, with significantly less T cells infiltrating into the aged ischemic hemisphere 7 days after 60 minute MCAO as assessed by Student's t test (p<0.01, n=4-10/group). (C) There was an effect of age (F(1,16)=p<0.0001), stroke (F(1,16)=p<0.0001) and an interaction between age and stroke (F(1,16)=p=0.0001) on the number of T cells (CD3+) as a proportion of all leukocytes infiltrating into the brain (CD45^hi cells in the brain) 72 hours after 90 minute MCAO as measured by 2-way ANOVA, with T cells making up a significantly greater proportion of infiltrating leukocytes in aged mice 7 days after 60 minute MCAO as assessed by Student's t test (p=0.05, n=4-10/group). Values are expressed as mean ± SEM. *, p≤0.05; **, p<0.01.