Research Paper Volume 8, Issue 4 pp 777—795

Figure 7. DAF-16 is required to maintain induced thermotolerance. Survival under 37°C heat stress and induced thermotolerance was equivalent between daf-16 and WT worms (A). Although daf-16 worms exhibit increased survival under heat stress one day after exposure to a mild 35°C heat stress for 2 hours (C), the induced thermotolerance is lost less than 3 days after the heat stress (C-F). daf-16 worms are able to activate the HSR, mitoUPR and ER-UPR in response to heat, oxidative stress and tunicamycin, respectively, to the same extent as WT worms at day 1 of adulthood (G). As in WT worms, the HSR in daf-16 worms, as measured by Phsp-16.2::GFP reporter activity, remains activated for at least 5 days following a mild 35°C heat stress for 2 hours (H). Also, similar to WT worms, the mitoUPR in daf-16 worms, as measured by Phsp-6::GFP reporter activity, remains active for at least 7 days following exposure to 4 mM paraquat for 1 day (oxidative stress). Error bars indicate SEM. *p<0.05, **p<0.01, ***p<0.001.