Research Paper Volume 8, Issue 5 pp 889—898

Figure 5. Autofluorescence in C. elegans does not increase in pro-oxidation conditions. (a) Supplementation of the media with Fe(III) in the form of 15 mM ferric ammonium citrate (FAC) shortens lifespan as previously reported [23]. Four replicate plates of 50 individuals each were analyzed for FAC and control conditions (light lines). The heavy line shows the overall average for each condition. (b) Despite shortening lifespan, iron supplementation does not increase autofluorescence levels in any channel investigated. While at several time-points t-test p-values indicate significant differences between the groups, the changes are relatively small in magnitude and are in all cases in the direction of less auto-fluorescence in the FAC group. Population variability is, however, somewhat increased at the last timepoint in the FAC group.