Research Paper Volume 8, Issue 5 pp 873—887

Figure 7. Insulin regulated the stability of PHLPP-1 in cardiomyocytes. Cardiomyocytes were treated with insulin (100 nM, 3 h). (A) Cardiac PHLPP-1 protein level. (B) Quantification of PHLPP-1mRNA levels. (C) The quantification of PHLPP-1 abundance in cardiomyocytes with or without insulin treatment. Cardiomyocytes were treated with CHX (20 μg/mL) alone or in combination with insulin (100 nM) and MG132 (10 μM) (*P < 0.05 vs. Insulin group. Values are means ± S.E., n = 5 per group). (D) Cardiomyocytes were subjected to H/R with or without insulin (100 nM) and MG132 (10 μM) treatment. The interaction between PHLPP-1 and β-TrCP detected by immunoprecipitation (IP). (E) Aged rats were subjected to 30 minutes of ischemia and followed by 30 minutes of reperfusion with or without insulin treatment (60 U/L, intravenous infusion at 4 mL/kg per h, beginning 5 min before reperfusion). The interaction between PHLPP-1 and β-TrCP detected by immunoprecipitation (IP).