Research Paper Volume 8, Issue 5 pp 873—887

Figure 5. PHLPP-1 knockdown alleviated ischemic injury of aged hearts in vivo. (A) The lentiviral plasmids encoding shRNAs for PHLPP-1 were injected into the left ventricular cavity of aged hearts while the aorta and pulmonary artery were cross-clamped for 50 s. Immunoblot analyses showing expressions of PHLPP-1 in young and aged hearts after 72 hours (infection with sh-PHL or sh-Con). (B) Young and aged hearts (infection with sh-PHL or sh-Con) were subjected to 30 minutes of in vivo ischemia and followed reperfusion. Representative sections of myocardial infarction and the percent of infarct size after 4 hours reperfusion. (C-F) Heart rate, left ventricular end diastolic pressure (LVEDP) and ±LVdp/dt determined by multi-channel physiological signal acquisition processing system after 2 hours reperfusion. (G) Cardiac function determined by echocardiography after 24 hours reperfusion. LVEF, left ventricular ejection fraction (*P < 0.05 vs. Sham; ^#P < 0.05 vs. Aged I/R+Ad-sh-Con. Values are means ± S.E., n = 5 per group).