Research Paper Volume 7, Issue 12 pp 1112—1128

Figure 3. Longevity of flies carrying mutations in both rpd3 and Thor (4E-BP). (A) Average expression of mRNA for the 4E-BP gene in two (yw) or three (Thor mutant) biological replicates isolated from the heads and thoraces of female flies at 10 days of age by Q-PCR. Black, +/+ controls, magenta, Thor^k07736/+. Graphs are plotted as the means +/− standard errors. (B) Mean life span of Thor^k0773/+ and +/+ control (yw background) flies, male and female, on 0.5N, 0.7N, 1.0N, and 1.5N food levels.(C, D) Mean life span of male (C) and female (D) rpd3^def/+, Thor^k0773 /+; rpd3^def/+ double mutants and +/+ control flies on 0.5N, 0.7N, 1.0N, and 1.5 food levels.