Research Perspective Volume 6, Issue 12 pp 1010—1018

Figure 2. Suppression of etoposide-induced senescence in RPE cells. (A) Reversibility potential (RP) in RPE cells [41] treated with 0.5 μg/ml etoposide (+Eto) in high cell density. After 2 day-treatment with etoposide (total 6 days in culture), cells were counted and re-plated in fresh medium at 1000/well in 24-well plates. After 7 days, cells were re-counted. Fold increase in cell numbers mean ± SD. (B) Protein per cell in regular (RD) versus high cell density (HD). RPE cells were plated at regular or high density. If indicated, cells were treated with 0.5 μg/ml etoposide (+Eto). After 2 days, cells were counted and lysed, protein amount was determined and protein (ng) amounts per cell was calculated. Data are mean ± SD. (C) Wounding. RPE cells were plated at high density. 0.5 μg/ml etoposide (+Eto) was added, if indicated “+”. Wounds were made in cell monolayer without changing the medium. After 3 days, cells were stained for beta-Gal.