Research Paper Volume 6, Issue 1 pp 48—57

Figure 4. BMAL1 deficiency results in increased mTORC1 signaling in the liver of TRF mice. Phosphorylation and expression of TORC1 downstream targets were analyzed by western blotting, image analyzing software was used to quantify the intensity of bands. For every experiment the value of maximum intensity of the band for wild type mice was set as 100, the intensity of bands for other time points and for Bmal1^−/− mice was normalized accordingly. Results represent average for 4 animals of each genotype for each time point. (a-c) Quantitative profiles of TORC1 downstream targets phosphorylation in the liver of wild type (black diamonds) and Bmal1^−/− (grey squares) TRF mice. (a) 4E-BP1 T37/46 phosphorylation. (b) S6K1 T389 phosphorylation; (c) S6K1 T421/424 phosphorylation. * - statistically significant difference between the genotypes. Food was provided at time point 0. Bars on the top of the figure represent light (open bars) and dark (black bars) parts of the day.