Research Paper Volume 5, Issue 4 pp 288—303

Figure 1. Absence of A-type lamins in iPSCs is associated with low levels of H3K4me3 on the LMNA promoter. (A) Immunofluorescence staining in normal and HGPS iPSCs with stem cell markers Tra-1-81 (green) and Tra-1-60 (red), and with lamin A/C antibody (red) and lamin B antibody (green). Nuclei are counterstained in blue with Hoechst 33342. Scale bar: 500 μm. (B) Western Blotting analysis with lamin A/C, lamin B, and GAPDH antibodies in normal and HGPS fibroblasts and iPSCs. Red filled triangle points to progerin. (C) Quantitative RT?PCR analysis showing the mRNA level of lamin A (left), progerin (middle), and lamin B (right) in normal and HGPS fibroblasts and iPSCs (n = 3). (D) ChIP-qPCR analysis of H3K4me3 at the LMNA gene promoter in a normal fibroblast line and three iPSC lines (n = 3). Cell lines: Normal (8470), HGPS or HGPS1 (164), and HGPS-2 (155).