Figure 2. Epidermal Thickness Decreases in Aging Flies. (A-D) TEM analysis of ventral pleura in w1118 controls. Bar, 2 μm. A, 1 d. B, 3 d. C, 14 d. D, 42 d. Red, epidermal boundaries; Blue, epidermal nuclei. c, cuticle; m, muscles. The presence of muscles underlying the epidermis varies with the precise plane of section and was not dependent on age.