Research Paper Volume 5, Issue 3 pp 155—173

Figure 9. Histoblotting of fCJD^Ins+sPrPSc and fCJD^Ins+rPrPSc. The nitrocellulose membranes blotted with tissue sections from the cerebellar cortex of fCJD^Ins+sPrPSc (A, C, and F) or fCJD^Ins+rPrPSc (B, D, and G) were developed with BCIP/NBT similar to classic IHC in A, B, E, F, and G while the membranes were developed with ECL Plus similar to Western blotting in C and D. PrP staining was detected in all blots without PK-treatment. After PK-treatment at either 10, 25, or 50 μg/ml, no PrP staining was detected in the samples from fCJD^Ins+sPrPSc (A and C), whereas PrP was detected in the samples from fCJD^Ins+rPrP (B and D). In contrast, PrP staining was detected in fCJD^Ins+sPrPSc (F) in addition to fCJD^Ins+rPrP (G) but not in normal controls after thermolysin that was reported to digest PrP^C only (E).