Research Paper Volume 5, Issue 3 pp 155—173

Figure 4. Comparison of oligomeric state of PrP in case 6 and other cases with rPrP^Sc by sucrose step gradient sedimentation. (A) Western blotting of PrP in individual fractions of sucrose gradient analysis of brain homogenate from case 3 with readily detectable rPrP^Sc. (B) Western blotting of PrP in individual fractions of sucrose gradient analysis of brain homogenate from case 6 with sPrP^Sc. C: Bar graph of PrP in individual fractions from three 144-bp insertion mutation cases with rPrP^Sc (average of PrP percentages from the three fCJD^Ins+rPrPSc cases) and case 6 with no rPrP^Sc. Blots were probed with 3F4 antibodies. D and E: PrP in individual fractions from cases 3 (D) and 6 (E) was detected by Western blotting after treatment with PK at 0.5 μg/ml. PrP was only detected in fCJD^Ins+rPrPSc but not in fCJD^Ins+sPrPSc.