Research Paper Volume 4, Issue 12 pp 923—931

Figure 3. Tyrosine hydroxylase levels correlates with p73 transactivation potential. N2a cells were transiently transfected with human TAp73β or shRNAs specific for exon 13; 48 hours later, cells were collected and processed. Semi-quantitative PCR showed that knock-down of exon 13 lead to a shift from α to β (A). Real-time PCR shows an increase of about 5 times in Th levels (B). In order to test efficiency of transfection, p73 levels were monitored (C). Experiment has been reproduced at least 3 times (data are represented as mean +/− SD). KD = knock-down, GAPDH = Glyceraldehyde 3-phosphate dehydro-genase, untr. = untreated. Experiment has been reproduced at least 3 times.