Figure 4. Influence of NF90 on the steady-state expression of SASP mRNAs. (A) Forty-eight h after transfecting WI-38 cells (pdl 30) with Ctrl siRNA or NF90 siRNA, total RNA was prepared and the levels of SASP mRNAs were quantified by RT-qPCR analysis (normalized to 18S rRNA levels). (B) Forty-eight h after transfecting IDH4 cells (+Dex) with Ctrl siRNA or NF90 siRNA, total RNA was prepared and the levels of SASP mRNAs were quantified by RT-qPCR analysis (normalized to 18S rRNA levels). Data represent the means +SD from three independent experiments.