Figure 5.CBS depletion increases susceptibility of HUVEC to homocysteine-induced senescenceCBS gene expression was inactivated by lentiviral shRNA vectors in early-passage HUVEC and HDF, as indicated, and homocysteine (Hcy) was added. Cells were counted after 72 h (panel A), and the percentage of SA-ß-gal positive cells was determined after 84 h (panel B). Data are represented as mean ± SE (n=3). Micrographs show representative pictures of cells stained for SA-ß-gal. ***: p<0.001, **: p<0.01, n.s.: not significant.
