Research Paper Volume 4, Issue 9 pp 636—647

Figure 1. Expression levels of miRNAs in the liver of Wrn^Dhel/Dhel mice compared to wild type mice and in the whole body of wild type and wrn-1(gk99) worms. (A) Total RNA from four mice (at three months of age) of the indicated genotype was used for the quantitative RT-PCR analyses. The levels of the indicated miRNAs in the Wrn^Dhel/Dhel mice are relative to the wild type (WT) animals. (B) Expression levels of miR-375 and miR-124 in the liver of four young (three months old) and four old (21 months old) wild type animals. The levels of the indicated miRNAs in the old wild type mice are relative to the young wild type animals. (C) Expression level of mir-124 in wild type and wrn-1(gk99) strains. Twenty-five 7-day old adult worms (post-larval L4 stage) of each strain were sorted and collected for total RNA extraction. The quantification of mir-124 were measured by quantitative RT-PCR (TaqMan assay) and compared with the levels found in wild type animals. (D) mir-124 expression levels in young and older adults wild type worms. All data were normalized by the quantification of the small nucleolar RNA (sn2841). The error bars represent the 95% confidence interval of three independent experiments. The P-values (unpaired Student's t-test) are indicated above each graph.