Figure 4. Comparison of miRNA biomarker pairs in plasma of Group 1 (30-50 years old, “CY”) and Group 2 (70-80 years old, “AMC”) individuals with normal cognitive functions. The concentrations of miRNA in plasma samples of Group1 (30-50 years old, CY) and Group2 (70-80 years old, AMC) donors with normal cognitive function, 20 samples in each group, were measured by RT-PCR and the ratio of various miRNA was calculated as 2−ΔCt × 100. See the legend to Fig.