Research Paper Volume 4, Issue 1 pp 28—39

Figure 5. Measurements on mtDNA of stably transfected PC3 cells. Cells were exposed to 10 nM rotenone for 6h, 72h, and 6g and recovery until 72h (6h+r). (A) copy number assessed by Real-time PCR. Data are expressed as ratio between the levels of ND1 and actin genes, and are the average value of three separate measurements ± st. dev. White columns: p53R72 expressing cells; black columns: p53P72 expressing cells. (B) Nested PCR specific for the 4,977 bp deletion. A representative experiment out of three is showed. 1,5: control; 2,6: 6h; 3,7: 6h+r; 4,8: 72h; Δ4,977: DNA from a subject carrying the 4,977 bp deletion. C: Long PCR for multiple mtDNA deletions. Numbers legend as in B; M: marker 1 Kb; C: non-transfected PC3 cells; N: DNA from PBMC of a young subject.