Figure 2. Typical undifferentiated and differentiating hESCs in culture. (A) A compact colony of proliferating pluripotent hESCs can be seen when cultured in defined medium on mouse embryonic fibroblasts. (B) Floating hEBs observed at 3 days after induction of differentiation. (C) Differentiating tissues, including cardiomyocytes, appear within adherent cultures at 48 hours after plating hEBs onto a gelatin-coated culture dish. Bar, 25 μm.