Research Paper Volume 3, Issue 4 pp 407—416

Figure 4. Dermabrasion stimulates fibroblast replication and suppresses senescence in geriatric dermis. (A) Skin biopsies described in Fig. 3B were stained with antibodies to 53BP1 and γH2AX. Senescent nuclei are indicated by white arrows, the basement membrane is designated by a dashed grey line, bar = 25 µm. (B) The density of fibroblasts in the papillary dermis was determined using the Nikon Elements Image Analysis software. Asterisk indicates statistical significance from control values (p = 0.0048), two-tailed t-test). (C) Sections of biopsies were stained with antibodies to Ki67. The percentage of Ki67(+) fibroblasts in the papillary dermis and the percentage of Ki67(+) keratinocytes in the basal layer of the epidermis were calculated using Nikon Elements image analysis software. Asterisks indicate statistical significance from control values (papillary dermis, p = 0.039; epidermis p = 0.058, two-tailed t-test).