Figure 2.Depletion of Ku70/80 in CCL75.1 cells induces transient p53 activationThe levels of p21, p53, and p53 phosphorylated at serine 15 were assessed by Western blotting in extracts prepared from CCL75.1 cells collected either before, or 3 to 6 days after addition of DOX. Tubulin serves as a loading control. Western blot analyses were performed with antibodies against p53, p53-phosphoserine15, p21, and tubulin.
