Figure 3.Effect of night vs. day RF and night vs. day IF on clock gene expression. Expression
of a representative clock gene mCry1 was measured in the liver of
C57BL mice during
ad libitum (AL), day and night RF, and day and night IF.Total
RNA extracted from liver tissue collected every 3 h around the circadian
cycle (mean ± SEM; n=3 for each time-point and each mouse group) was
reverse transcribed and analyzed by quantitative real time PCR. Clock gene levels
were normalized using Gapdh as the reference gene. The grey and
black bars designate the subjective light and dark cycles, respectively.
