Review Volume 2, Issue 1 pp 7—27

Figure 3. Effect of night vs. day RF and night vs. day IF on clock gene expression. Expression of a representative clock gene mCry1 was measured in the liver of C57BL mice during ad libitum (AL), day and night RF, and day and night IF.Total RNA extracted from liver tissue collected every 3 h around the circadian cycle (mean ± SEM; n=3 for each time-point and each mouse group) was reverse transcribed and analyzed by quantitative real time PCR. Clock gene levels were normalized using Gapdh as the reference gene. The grey and black bars designate the subjective light and dark cycles, respectively.