Figure 2.Conditional over-expression of wild-type p53 trans-genes using Geneswitch system.
All flies were the progeny of either Oregon R
control (A) or p53-WT transgenic strain (B, C) crossed
to the tissue-general Geneswitch driver Act-GS-255B. The flies were
cultured in the presence and absence of drug, as larvae or adults, as
indicated: M = males, F = females, + indicates culture in presence of
drug, - indicates culture in absence of drug. The number of flies in each
group are indicated in parentheses. (A, B) Blue diamonds
indicate male adults plus drug, pink squares indicate male adults minus
drug, orange triangles indicate female adult plus drug, turquoise x
indicates female adults minus drug. (A) Control flies, progeny of
Oregon R wild-type and Act-GS-255B. (B) p53 wild-type
transgene over-expression. Note male larvae plus drug produced no adult
flies, whereas female larvae plus drug produced only three escapers. (C)
Titration of p53 wild-type over-expression during female larval
development and effect on subsequent adult life span. EtOH indicates the
ethanol solvent for the drug alone (vector control, indi-cated with light
blue diamonds). Repeats of the titration experiments, including data for
males are presented in Supplementary Figure 1.
