Priority Research Paper Volume 1, Issue 11 pp 903—936

Figure 2. Conditional over-expression of wild-type p53 trans-genes using Geneswitch system. All flies were the progeny of either Oregon R control (A) or p53-WT transgenic strain (B, C) crossed to the tissue-general Geneswitch driver Act-GS-255B. The flies were cultured in the presence and absence of drug, as larvae or adults, as indicated: M = males, F = females, + indicates culture in presence of drug, - indicates culture in absence of drug. The number of flies in each group are indicated in parentheses. (A, B) Blue diamonds indicate male adults plus drug, pink squares indicate male adults minus drug, orange triangles indicate female adult plus drug, turquoise x indicates female adults minus drug. (A) Control flies, progeny of Oregon R wild-type and Act-GS-255B. (B) p53 wild-type transgene over-expression. Note male larvae plus drug produced no adult flies, whereas female larvae plus drug produced only three escapers. (C) Titration of p53 wild-type over-expression during female larval development and effect on subsequent adult life span. EtOH indicates the ethanol solvent for the drug alone (vector control, indi-cated with light blue diamonds). Repeats of the titration experiments, including data for males are presented in Supplementary Figure 1.