Research Paper Volume 1, Issue 1 pp 122—130

Figure 1. Lentiviral expression of mTRF2 and GFP-mTRF2. (A) Schematic of transgene constructs. GFP, mouse TRF2 (mTRF2) and a GFP-mTRF2 fusion were cloned into a lentiviral vector system [35] under the control of a CAG promoter [38]. (B) Indirect immunofluorescence of 3T3 cells. 3T3 control cells (top panel), 3T3 cells transfected with GFP-mTRF2 and cells transfected with the mTRF2 construct were stained with antibodies against mTRF1 or mTRF2. GFP was visualized by autofluorescence. DNA has been stained with DAPI, and the merge of the red, green and blue channels has been provided on the right. (C) Indirect immunofluorescence of CD45.1 donor bone marrow cells. Cells were infected with GFP-mTRF2 expressing lentiviruses and GFP-mTRF2 was visualized by GFP autofluorescence. TRF1 was detected by a mTRF1 specific antibody, the DNA was counterstained with DAPI And the merge of the three colors is indicated.