Figure 7.SIRT6 is not required for DNA double-strand break rejoining in a cell-free system.(a) Western analysis showing immunodepletion
of SIRT6 from HeLa cell nuclear extracts (NE) with anti-SIRT6 antibodies.
Mock-depleted control nuclear extracts were generated using the protein
A/G-sepharose beads alone. (b) Ethidium bromide stain (inverted
image) of DNA products of cell-free DSB rejoining assay. Reactions
contained linearized pUC19 plasmid DNA fragments and SIRT6- or mock-
depleted nuclear extracts. No difference in ligated species is observed
between SIRT6-depleted and mock-depleted reactions.
